ABSTRACT
<p><b>OBJECTIVE</b>To optimize the protocols for isolation and culture of mesenchymal stem cells from rat bone marrow (BMSCs).</p><p><b>METHODS</b>BMSCs were isolated by adherence to plastic with frequent medium change and reduced trypsinization time. The cell growth curves were drawn and the surface markers of BMSCs were detected by flow cytometry. The cells were induced to differentiate into osteogenic, adipogenic, hepatic and cholic lineages.</p><p><b>RESULTS</b>The cells isolated using this method were positive for CD29, CD44, and CD90 and negative for the hematopoietic surface markers CD45. The osteogenic and adipogenic differentiation of the BMSCs was verified by alkaline phosphatase staining, Alizarin red staining and Oil red staining. The cell subcultures up to passage 10 maintained capacities of differentiation into osteogenic and adipogenic lineages. The BMSCs induced with sequential addition of growth factors, cytokines and hormones differentiated into cells expressing hepatocyte- and cholangiocyte-specific markers.</p><p><b>CONCLUSION</b>The optimized method allows efficient isolation of homogenous populations of MSCs from rat bone marrow, which can be induced into multiple cell lineages.</p>
Subject(s)
Animals , Rats , Cell Culture Techniques , Cell Differentiation , Cell Proliferation , Cell Separation , Flow Cytometry , Mesenchymal Stem Cells , Cell BiologyABSTRACT
OBJECTIVE@#To investigate the advantages of nitric acid digestion method and its differences with the traditional method.@*METHODS@#Ethanol was used to fully fix the testing sample. About 80-100 g of the testing samples were cut into pieces and digested with nitric acid. It was then centrifuged and washed to remove organic components. Smears were prepared and examined under the light microscope.@*RESULTS@#The diatom had been identified with clear striations, counted conveniently and classified easily.@*CONCLUSION@#The improved nitric acid digestion method is not only simple with a higher successful rate of detection, but also can prevent interference from contamination. It can improve the stability of the experimental results, avoid harm to human and environment, and provide higher safety in the course of experiment.
Subject(s)
Humans , Autopsy , Diatoms/isolation & purification , Drowning , Forensic Pathology , Kidney/metabolism , Liver/metabolism , Lung/metabolism , Nitric Acid/chemistry , Postmortem Changes , Tissue Fixation/methodsABSTRACT
Comrade ZHU Lian is a main founder of Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, and she is the first to learn TCM for a physician of western medicine. The present paper looks back the works of comrade ZHU in acupuncture and moxibustion cause. Her main contributions are learning acupuncture and moxibustion medicine, popularizing acupuncture and moxibustion therapy; being concerned acupuncture and moxibustion cause, establishing organization of acupuncture and moxibustion research, more early conducting international exchanges of acupuncture and moxibustion; paying attention to clinical practice, proposing scientific research of acupuncture and moxibustion; writing New Acupuncture and Moxibustion Science, passing on later generations.
Subject(s)
Acupuncture , China , History, 20th CenturyABSTRACT
AimTo study the effects of glipizide and met formin on the serum IGF-1,IGF-2 in patients with type Ⅱ diabetes mellitus; Methods The effect of glipizide(n ＝ 40) and metformin(n ＝ 25) on serum IGF-1, IGF-2 in patients with type Ⅱ diabetes mellitus were compared with self- controlled study. Results In metformin-treated patients ,there were not significantly changes in fasting IGF-1 and IGF-2 concentrations, In glipizide-treated patients, there were markedly increased IGF-1 concentrations(181.8+ 104.5) vs (209.0+ 88.2) ng· ml-1(P＜0.05) while serum IGF-2 was not change. There was a significant reduction of blood glucose in two groups at the end of treatment(both P＜0.01), but C-peptide level was markedly increased(P＜0.05) only in glipizide-treatedpatients.Conclusion The changes of IGF-1 is markedly different between metformin-treated and glipizide-treated patients with type Ⅱ diabetes mellitus.
ABSTRACT
Aim To study the effects of glipizide and metformin on the serum IGF_1,IGF_2 in patients with type Ⅱ diabetes mellitus;Methods The effect of glipizide(n=40) and metformin(n=25) on serum IGF_1,IGF_2 in patients with type Ⅱ diabetes mellitus were compared with self_ controlled study.Results In metformin_treated patients ,there were not significantly changes in fasting IGF_1 and IGF_2 concentrations,In glipizide_treated patients,there were markedly increased IGF_1 concentrations (181.8?104.5) vs (209.0?88.2) ng?ml-1(P